High-throughput genotyping of high-homology mutant mouse strains by next-generation sequencing

Genotyping of knockout alleles in mice is commonly performed by end-point PCR or gene-specific/universal cassette qPCR. Both have advantages and limitations in terms of assay design and interpretation of results. As an alternative method for high-throughput genotyping, we investigated next generatio...

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Udgivet i:Methods
Main Authors: Gleeson, Diane, Sethi, Debarati, Platte, Radka, Burvill, Jonathan, Barrett, Daniel, Akhtar, Shaheen, Bruntraeger, Michaela, Bottomley, Joanna, Mouse Genetics Project, Sanger, Bussell, James, Ryder, Edward
Format: Artigo
Sprog:Inglês
Udgivet: Academic Press 2021
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8205115/
https://ncbi.nlm.nih.gov/pubmed/33096238
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2020.10.011
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