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Quantification of Colibactin-associated Genotoxicity in HeLa Cells by In Cell Western (ICW) Using γ-H2AX as a Marker
The genotoxin colibactin is produced by several species of Enterobacteriaceae. This genotoxin induces DNA damage, cell cycle arrest, senescence and death in eukaryotic cells ( Nougayrède et al., 2006 ; Taieb et al., 2016 ). Here we describe a method to quantify the genotoxicity of bacteria producing...
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| Publicado no: | Bio Protoc |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Bio-Protocol
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8203971/ https://ncbi.nlm.nih.gov/pubmed/34179287 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2771 |
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