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Detection of Protein Interactions in the Cytoplasm and Periplasm ofEscherichia coli by Förster Resonance Energy Transfer
This protocol was developed to qualitatively and quantitatively detect protein-protein interactions in Escherichia coli by Förster Resonance Energy Transfer (FRET). The described assay allows for the previously impossible in vivo screening of periplasmic protein-protein interactions. In FRET, excita...
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| Veröffentlicht in: | Bio Protoc |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Bio-Protocol
2018
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8203949/ https://ncbi.nlm.nih.gov/pubmed/34179246 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2697 |
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