Detection of Protein Interactions in the Cytoplasm and Periplasm ofEscherichia coli by Förster Resonance Energy Transfer

This protocol was developed to qualitatively and quantitatively detect protein-protein interactions in Escherichia coli by Förster Resonance Energy Transfer (FRET). The described assay allows for the previously impossible in vivo screening of periplasmic protein-protein interactions. In FRET, excita...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:Bio Protoc
Autori principali: Meiresonne, Nils Y., Alexeeva, Svetlana, van der Ploeg, René, den Blaauwen, Tanneke
Natura: Artigo
Lingua:Inglês
Pubblicazione: Bio-Protocol 2018
Soggetti:
Methods Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC8203949/
https://ncbi.nlm.nih.gov/pubmed/34179246
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2697
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