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Targeted Genome Editing of Virulent Phages Using CRISPR-Cas9
This protocol describes a straightforward method to generate specific mutations in the genome of strictly lytic phages. Briefly, a targeting CRISPR-Cas9 system and a repair template suited for homologous recombination are provided inside a bacterial host, here the Gram-positive model Lactococcus lac...
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| Publicado no: | Bio Protoc |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Bio-Protocol
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8203862/ https://ncbi.nlm.nih.gov/pubmed/34179229 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2674 |
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