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Introduction of temperature-sensitive helper and donor plasmids into Bac-to-Bac baculovirus expression systems
In the baculovirus shuttle vector (bacmid) system, a helper plasmid and a donor plasmid are employed to insert heterologous genes into a cloned baculovirus genome via Tn7 transposition in Escherichia coli. The helper and donor plasmids are usually cotransfected with constructed bacmids into insect c...
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| Publicado no: | Virol Sin |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Wuhan Institute of Virology, CAS
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8200906/ https://ncbi.nlm.nih.gov/pubmed/26494478 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12250-015-3638-8 |
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