Introduction of temperature-sensitive helper and donor plasmids into Bac-to-Bac baculovirus expression systems

In the baculovirus shuttle vector (bacmid) system, a helper plasmid and a donor plasmid are employed to insert heterologous genes into a cloned baculovirus genome via Tn7 transposition in Escherichia coli. The helper and donor plasmids are usually cotransfected with constructed bacmids into insect c...

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Bibliographic Details
Published in:Virol Sin
Main Authors: Huang, Zhihong, Li, Ao, Pan, Mengjia, Wu, Wenbi, Yuan, Meijin, Yang, Kai
Format: Artigo
Language:Inglês
Published: Wuhan Institute of Virology, CAS 2015
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC8200906/
https://ncbi.nlm.nih.gov/pubmed/26494478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12250-015-3638-8
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