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High-Throughput Sequencing of Phage Display Libraries Reveals Parasitic Enrichment of Indel Mutants Caused by Amplification Bias

The combination of phage display technology with high-throughput sequencing enables in-depth analysis of library diversity and selection-driven dynamics. We applied short-read sequencing of the mutagenized region on focused display libraries of two homologous nucleic acid modification eraser protein...

詳細記述

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書誌詳細
出版年:Int J Mol Sci
主要な著者: Plessers, Sander, Van Deuren, Vincent, Lavigne, Rob, Robben, Johan
フォーマット: Artigo
言語:Inglês
出版事項: MDPI 2021
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC8197208/
https://ncbi.nlm.nih.gov/pubmed/34073702
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms22115513
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