Dissociation of microdissected mouse brain tissue for artifact free single-cell RNA sequencing

Single-cell RNA sequencing (scRNA-seq) provides the transcriptome of individual cells and addresses previously intractable problems including the central nervous system’s transcriptional responses during health and disease. However, dissociating brain cells is challenging and induces artificial tran...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Liu, Lu, Besson-Girard, Simon, Ji, Hao, Gehring, Katrin, Bulut, Buket, Kaya, Tuğberk, Usifo, Fumere, Simons, Mikael, Gokce, Ozgun
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8196224/
https://ncbi.nlm.nih.gov/pubmed/34159323
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100590
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