Dissociation of microdissected mouse brain tissue for artifact free single-cell RNA sequencing

Single-cell RNA sequencing (scRNA-seq) provides the transcriptome of individual cells and addresses previously intractable problems including the central nervous system’s transcriptional responses during health and disease. However, dissociating brain cells is challenging and induces artificial tran...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Liu, Lu, Besson-Girard, Simon, Ji, Hao, Gehring, Katrin, Bulut, Buket, Kaya, Tuğberk, Usifo, Fumere, Simons, Mikael, Gokce, Ozgun
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2021
Fag:
Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8196224/
https://ncbi.nlm.nih.gov/pubmed/34159323
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100590
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