Protocol to extract actively translated mRNAs from mouse hypothalamus by translating ribosome affinity purification

Here, we present an in-depth protocol for extracting ribosome-bound mRNAs in low-abundance cells of hypothalamic nuclei. mRNAs are extracted from the micropunched tissue using refined translating ribosome affinity purification. Isolated RNAs can be used for sequencing or transcript quantification. T...

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Enregistré dans:
Détails bibliographiques
Publié dans:STAR Protoc
Auteurs principaux: Han, Xingfa, Burger, Laura L., Garcia-Galiano, David, Moenter, Suzanne M., Myers, Martin G., Olson, David P., Elias, Carol F.
Format: Artigo
Langue:Inglês
Publié: Elsevier 2021
Sujets:
Protocol
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8196219/
https://ncbi.nlm.nih.gov/pubmed/34159322
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100589
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