Quantification of mRNA ribosomal engagement in human neurons using parallel translating ribosome affinity purification (TRAP) and RNA sequencing

Translation regulation is a fundamental step in gene regulation with critical roles in neurodevelopment. Here, we describe three protocols to calculate the ribosomal-engagement levels of the transcriptome from in vitro-derived neuronal cells. The protocols described here include enrichment of in vit...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:STAR Protoc
Egile Nagusiak: Rodrigues, Deivid Carvalho, Mufteev, Marat, Ellis, James
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Elsevier 2020
Gaiak:
Protocol
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC7753199/
https://ncbi.nlm.nih.gov/pubmed/33364619
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100229
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