Isolation of Nuclei from Mammalian Cells and Tissues for Single-Nucleus Molecular Profiling

Both single-cell RNA sequencing (scRNAseq) and single-nucleus RNA sequencing (snRNAseq) can be used to characterize the transcriptional profile of individual cells, and based on these transcriptional profiles, help define the cell type distribution in mixed cell populations. However, scRNAseq analys...

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Publicado en:Curr Protoc
Autores principales: Nadelmann, Emily R., Gorham, Joshua M., Reichart, Daniel, Delaughter, Daniel M., Wakimoto, Hiroko, Lindberg, Eric K., Litviňukova, Monika, Maatz, Henrike, Curran, Justin J., Gutierrez, Daniela Ischiu, Hübner, Norbert, Seidman, Christine E., Seidman, J. G.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2021
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC8191490/
https://ncbi.nlm.nih.gov/pubmed/34043278
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpz1.132
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