Using the dCas9-KRAB system to repress gene expression in hiPSC-derived NGN2 neurons

We describe a CRISPR inhibition (CRISPRi) protocol to repress endogenous gene expression (e.g., ATP6V1A) in human induced pluripotent stem cell-derived NGN2-induced glutamatergic neurons. CRISPRi enables efficient and precise gene repression of one or multiple target genes via delivering gRNA(s) to...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Li, Aiqun, Cartwright, Samuel, Yu, Alex, Ho, Seok-Man, Schrode, Nadine, Deans, P.J. Michael, Matos, Marliette R., Garcia, Meilin Fernandez, Townsley, Kayla G., Zhang, Bin, Brennand, Kristen J.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8188621/
https://ncbi.nlm.nih.gov/pubmed/34151300
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100580
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