Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions

We describe the extended endothelial cell culture method (EECM) for the differentiation of human pluripotent stem cells (hPSCs) into brain microvascular endothelial cell (BMEC)-like cells. EECM-BMEC-like cells resemble primary human BMECs in morphology, molecular junctional architecture, and diffusi...

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Detalles Bibliográficos
Publicado en:STAR Protoc
Main Authors: Nishihara, Hideaki, Gastfriend, Benjamin D., Kasap, Pelin, Palecek, Sean P., Shusta, Eric V., Engelhardt, Britta
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2021
Assuntos:
Protocol
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC8188617/
https://ncbi.nlm.nih.gov/pubmed/34151293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100563
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