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Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides

Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequent HLA i...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Chatzileontiadou, Demetra S.M., Szeto, Christopher, Jayasinghe, Dhilshan, Gras, Stephanie
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8188458/
https://ncbi.nlm.nih.gov/pubmed/34124695
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100635
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