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Detection of microcrystals for CryoEM
Here we present a strategy to identify microcrystals from initial protein crystallization screen experiments and to optimize diffraction quality of those crystals using negative stain transmission electron microscopy (TEM) as a guiding technique. The use of negative stain TEM allowed visualization a...
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| 發表在: | Methods Mol Biol |
|---|---|
| Main Authors: | , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2021
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8188421/ https://ncbi.nlm.nih.gov/pubmed/33368010 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0966-8_14 |
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