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One‐step selective affinity purification and immobilization of His‐tagged enzyme by recyclable magnetic nanoparticles
The NiFe(2)O(4) magnetic nanoparticles (NF‐MNPs) were prepared for one‐step selective affinity purification and immobilization of His‐tagged recombinant glucose dehydrogenase (GluDH). The prepared nanoparticles were characterized by a Fourier‐transform infrared spectrophotometer and microscopy. The...
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| Publicado no: | Eng Life Sci |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
John Wiley and Sons Inc.
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8182278/ https://ncbi.nlm.nih.gov/pubmed/34140847 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elsc.202000093 |
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