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One‐step selective affinity purification and immobilization of His‐tagged enzyme by recyclable magnetic nanoparticles
The NiFe(2)O(4) magnetic nanoparticles (NF‐MNPs) were prepared for one‐step selective affinity purification and immobilization of His‐tagged recombinant glucose dehydrogenase (GluDH). The prepared nanoparticles were characterized by a Fourier‐transform infrared spectrophotometer and microscopy. The...
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| Veröffentlicht in: | Eng Life Sci |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
John Wiley and Sons Inc.
2021
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8182278/ https://ncbi.nlm.nih.gov/pubmed/34140847 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elsc.202000093 |
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