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Polymer Brush-Modified Magnetic Nanoparticles for His-Tagged Protein Purification
Growth of poly(2-hydroxyethyl methacrylate) brushes on magnetic nanoparticles and subsequent brush functionalization with nitrilotriacetate-Ni(2+) yield magnetic beads that selectively capture polyhistidine-tagged (His-tagged) protein directly from cell extracts. Transmission electron microscopy, FT...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2011
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3153590/ https://ncbi.nlm.nih.gov/pubmed/21338107 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/la1050404 |
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