Immunofluorescent staining of cancer spheroids and fine-needle aspiration-derived organoids

Our organoid generation technique has allowed for the development of downstream organoid applications. Here, we detail an accessible, straightforward protocol for immunofluorescent staining and imaging of thyroid cancer organoids, particularly those with tumor de-differentiation. Immunofluorescence...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Bergdorf, Kensey N., Phifer, Courtney J., Bechard, Matthew E., Lee, Mason A., McDonald, Oliver G., Lee, Ethan, Weiss, Vivian L.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8182106/
https://ncbi.nlm.nih.gov/pubmed/34136836
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100578
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