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High-throughput drug screening of fine-needle aspiration-derived cancer organoids

Generation of fine-needle aspiration (FNA)-derived cancer organoids has allowed us to develop a number of downstream applications. In this protocol, we start with organoids cultured in a semi-solid format. We dissociate organoids into single cells and then plate in a 384-well format for high-through...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Bergdorf, Kensey, Phifer, Courtney, Bharti, Vijaya, Westover, David, Bauer, Joshua, Vilgelm, Anna, Lee, Ethan, Weiss, Vivian
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757655/
https://ncbi.nlm.nih.gov/pubmed/33377106
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100212
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