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Single-nucleotide resolution of N(6)-adenine methylation sites in DNA and RNA by nitrite sequencing
A single-nucleotide resolution sequencing method of N(6)-adenine methylation sites in DNA and RNA is described. Using sodium nitrite under acidic conditions, chemoselective deamination of unmethylated adenines readily occurs, without competing deamination of N(6)-adenine sites. The deamination of ad...
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| Vydáno v: | Chem Sci |
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| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
The Royal Society of Chemistry
2020
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8179008/ https://ncbi.nlm.nih.gov/pubmed/34163791 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/d0sc03509b |
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