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An in vivo selection system with tightly regulated gene expression enables directed evolution of highly efficient enzymes
In vivo selection systems are powerful tools for directed evolution of enzymes. The selection pressure of the systems can be tuned by regulating the expression levels of the catalysts. In this work, we engineered a selection system for laboratory evolution of highly active enzymes by incorporating a...
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| Veröffentlicht in: | Sci Rep |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Nature Publishing Group UK
2021
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8175713/ https://ncbi.nlm.nih.gov/pubmed/34083677 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-91204-4 |
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