Ammonia-based enrichment and long-term propagation of zone I hepatocyte-like cells

Ammonia has a cytotoxic effect and can therefore be used as a selection agent for enrichment of zone I hepatocytes. However, it has not yet been determined whether ammonia-treated hepatocyte-like cells are able to proliferate in vitro. In this study, we employed an ammonia selection strategy to puri...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Tsuneishi, Ruri, Saku, Noriaki, Miyata, Shoko, Akiyama, Saeko, Javaregowda, Palaksha Kanive, Ite, Kenta, Takashima, Nagisa, Toyoda, Masashi, Kimura, Tohru, Kuroda, Masahiko, Nakazawa, Atsuko, Kasahara, Mureo, Nonaka, Hidenori, Kamiya, Akihide, Kiyono, Tohru, Yamauchi, Junji, Umezawa, Akihiro
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2021
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8166824/
https://ncbi.nlm.nih.gov/pubmed/34059723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-90708-3
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