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Mechanically tightening, untying and retying a protein trefoil knot by single-molecule force spectroscopy
Knotted conformation is one of the most surprising topological features found in proteins, and understanding the folding mechanism of such knotted proteins remains a challenge. Here, we used optical tweezers (OT) to investigate the mechanical unfolding and folding behavior of a knotted protein Esche...
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| Veröffentlicht in: | Chem Sci |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
The Royal Society of Chemistry
2020
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8162576/ https://ncbi.nlm.nih.gov/pubmed/34123232 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/d0sc02796k |
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