Tightening the Knot in Phytochrome by Single-Molecule Atomic Force Microscopy

A growing number of proteins have been shown to adopt knotted folds. Yet the biological roles and biophysical properties of these knots remain poorly understood. We used protein engineering and atomic force microscopy to explore the single-molecule mechanics of the figure-eight knot in the chromopho...

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Bibliographic Details
Main Authors: Bornschlögl, Thomas, Anstrom, David M., Mey, Elisabeth, Dzubiella, Joachim, Rief, Matthias, Forest, Katrina T.
Format: Artigo
Language:Inglês
Published: The Biophysical Society 2009
Subjects:
Protein
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2717258/
https://ncbi.nlm.nih.gov/pubmed/19217867
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2008.11.012
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