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Boosting Detection of Low-Abundance Proteins in Thermal Proteome Profiling Experiments by Addition of an Isobaric Trigger Channel to TMT Multiplexes

[Image: see text] The study of low-abundance proteins is a challenge to discovery-based proteomics. Mass spectrometry (MS) applications, such as thermal proteome profiling (TPP), face specific challenges in the detection of the whole proteome as a consequence of the use of nondenaturing extraction b...

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Vydáno v:Anal Chem
Hlavní autoři: Peck Justice, Sarah A., McCracken, Neil A., Victorino, José F., Qi, Guihong D., Wijeratne, Aruna B., Mosley, Amber L.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Chemical Society 2021
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8153406/
https://ncbi.nlm.nih.gov/pubmed/33908254
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.1c00012
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