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DNA methylation analysis: speedup of bisulfite-mediated deamination of cytosine in the genomic sequencing procedure

Understanding the biological consequences of DNA methylation is a current focus of intensive studies. A standard method for analyzing the methylation at position 5 of cytosines in genomic DNA involves chemical modification of the DNA with bisulfite, followed by PCR amplification and sequencing. Bisu...

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Detalhes bibliográficos
Publicado no:Proc Jpn Acad Ser B Phys Biol Sci
Main Authors: Hayatsu, Hikoya, Negishi, Kazuo, Shiraishi, Masahiko
Formato: Artigo
Idioma:Inglês
Publicado em: The Japan Academy 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8153359/
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