An Optimized Preparation Method for Long ssDNA Donors to Facilitate Quick Knock-In Mouse Generation

Fluorescent reporter mouse lines and Cre/Flp recombinase driver lines play essential roles in investigating various molecular functions in vivo. Now that applications of the CRISPR/Cas9 genome-editing system to mouse fertilized eggs have drastically accelerated these knock-in mouse generations, the...

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Publicado no:Cells
Main Authors: Inoue, Yukiko U., Morimoto, Yuki, Yamada, Mayumi, Kaneko, Ryosuke, Shimaoka, Kazumi, Oki, Shinji, Hotta, Mayuko, Asami, Junko, Koike, Eriko, Hori, Kei, Hoshino, Mikio, Imayoshi, Itaru, Inoue, Takayoshi
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2021
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8147208/
https://ncbi.nlm.nih.gov/pubmed/33946570
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/cells10051076
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