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A Systematic Comparison of Protocols for Recovery of High-Quality RNA from Human Islets Extracted by Laser Capture Microdissection

The isolation of high-quality RNA from endocrine pancreas sections represents a considerable challenge largely due to the high ribonuclease levels. Laser capture microdissection (LCM) of mammalian islets, in association with RNA extraction protocols, has emerged as a feasible approach to characteriz...

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Détails bibliographiques
Publié dans:Biomolecules
Auteurs principaux: Cefalo, Chiara M. A., Mezza, Teresa, Giaccari, Andrea, Kulkarni, Rohit N.
Format: Artigo
Langue:Inglês
Publié: MDPI 2021
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8144988/
https://ncbi.nlm.nih.gov/pubmed/33922227
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/biom11050625
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