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Refinement of 16S rRNA gene analysis for low biomass biospecimens

High-throughput phylogenetic 16S rRNA gene analysis has permitted to thoroughly delve into microbial community complexity and to understand host-microbiota interactions in health and disease. The analysis comprises sample collection and storage, genomic DNA extraction, 16S rRNA gene amplification, h...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Villette, Remy, Autaa, Gaelle, Hind, Sophie, Holm, Johanna B., Moreno-Sabater, Alicia, Larsen, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8144411/
https://ncbi.nlm.nih.gov/pubmed/34031485
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-90226-2
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