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In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegeneration

Fluorescent staining of newly transcribed RNA via metabolic labelling with 5-ethynyluridine (EU) and click chemistry enables visualisation of changes in transcription, such as in conditions of cellular stress. Here, we tested whether EU labelling can be used to examine transcription in vivo in mouse...

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Détails bibliographiques
Publié dans:Acta Neuropathol Commun
Auteurs principaux: van’t Sant, Lisanne J., White, Joshua J., Hoeijmakers, Jan H. J., Vermeij, Wilbert P., Jaarsma, Dick
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2021
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8139001/
https://ncbi.nlm.nih.gov/pubmed/34020718
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s40478-021-01200-y
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