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Expression and purification of soluble and active human enterokinase light chain in Escherichia coli
Human enterokinase light chain (hEK(L)) specifically cleaves the sequence (Asp)(4)-Lys↓X (D(4)K), making this a frequently used enzyme for site-specific cleavage of recombinant fusion proteins. However, hEK(L) production from Escherichia coli is limited due to intramolecular disulphide bonds. Here,...
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| Publicado no: | Biotechnol Rep (Amst) |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8134707/ https://ncbi.nlm.nih.gov/pubmed/34026576 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.btre.2021.e00626 |
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