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Expression and purification of soluble and active human enterokinase light chain in Escherichia coli
Human enterokinase light chain (hEK(L)) specifically cleaves the sequence (Asp)(4)-Lys↓X (D(4)K), making this a frequently used enzyme for site-specific cleavage of recombinant fusion proteins. However, hEK(L) production from Escherichia coli is limited due to intramolecular disulphide bonds. Here,...
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| 發表在: | Biotechnol Rep (Amst) |
|---|---|
| Main Authors: | , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Elsevier
2021
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8134707/ https://ncbi.nlm.nih.gov/pubmed/34026576 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.btre.2021.e00626 |
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