A high-efficiency method for site-directed mutagenesis of large plasmids based on large DNA fragment amplification and recombinational ligation

Site-directed mutagenesis for large plasmids is a difficult task that cannot easily be solved by the conventional methods used in many laboratories. In this study, we developed an effective method for Site-directed Mutagenesis for Large Plasmids (SMLP) based on a PCR technique. The SMLP method combi...

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Vydáno v:Sci Rep
Hlavní autoři: Zhang, Kewei, Yin, Xiaomei, Shi, Kaituo, Zhang, Shihua, Wang, Juan, Zhao, Shasha, Deng, Huan, Zhang, Cheng, Wu, Zihui, Li, Yuan, Zhou, Xiangyu, Deng, Wensheng
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2021
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8129136/
https://ncbi.nlm.nih.gov/pubmed/34001951
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-89884-z
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