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Optimizing expression of a single copy transgene in C. elegans

The utility of single copy transgenic insertions in C. elegans is often limited by low expression. We examined the effects of modifying the trans-splicing signal, the Kozak ribosome binding site, the N-terminal amino acid of the reporter and the 3′ UTR sequences on the expression level of a mec-4 pr...

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Bibliografiska uppgifter
I publikationen:MicroPubl Biol
Huvudupphovsmän: Dour, Scott, Nonet, Michael
Materialtyp: Artigo
Språk:Inglês
Publicerad: Caltech Library 2021
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC8116934/
https://ncbi.nlm.nih.gov/pubmed/33997659
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.17912/micropub.biology.000394
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