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Quantifying Drp1-Mediated Mitochondrial Fission by Immunostaining in Fixed Cells
Dynamin-like protein 1 (Drp1) is the master regulator of mitochondrial fission. Drp1 translocates from the cytosol to the mitochondrial outer membrane to execute the scission process. Here we describe an immunofluorescence-based method to measure the mitochondrial translocation of Drp1 and quantify...
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| Publié dans: | Methods Mol Biol |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2020
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8106401/ https://ncbi.nlm.nih.gov/pubmed/32529373 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0676-6_15 |
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