Novel RT-ddPCR assays for measuring the levels of subgenomic and genomic SARS-CoV-2 transcripts

The replication of SARS-CoV-2 and other coronaviruses depends on transcription of negative-sense RNA intermediates that serve as the templates for the synthesis of positive-sense genomic RNA (gRNA) and multiple different subgenomic mRNAs (sgRNAs) encompassing fragments arising from discontinuous tra...

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Detalhes bibliográficos
Publicado no:Methods
Main Authors: Telwatte, Sushama, Martin, Holly Anne, Marczak, Ryan, Fozouni, Parinaz, Vallejo-Gracia, Albert, Kumar, G. Renuka, Murray, Victoria, Lee, Sulggi, Ott, Melanie, Wong, Joseph K., Yukl, Steven A.
Formato: Artigo
Idioma:Inglês
Publicado em: Academic Press 2022
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8105137/
https://ncbi.nlm.nih.gov/pubmed/33882362
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2021.04.011
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