Whole lung tissue is the preferred sampling method for amplicon-based characterization of murine lung microbiota

BACKGROUND: Low-biomass microbiome studies (such as those of the lungs, placenta, and skin) are vulnerable to contamination and sequencing stochasticity, which obscure legitimate microbial signal. While human lung microbiome studies have rigorously identified sampling strategies that reliably captur...

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Bibliografske podrobnosti
izdano v:Microbiome
Main Authors: Baker, Jennifer M., Hinkle, Kevin J., McDonald, Roderick A., Brown, Christopher A., Falkowski, Nicole R., Huffnagle, Gary B., Dickson, Robert P.
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2021
Teme:
Research
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC8101028/
https://ncbi.nlm.nih.gov/pubmed/33952355
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s40168-021-01055-4
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