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An optimized two-step chromatin immunoprecipitation protocol to quantify the associations of two separate proteins and their common target DNA
Sequential chromatin immunoprecipitation (ChIP) is commonly used to investigate DNA-protein and protein-protein interactions to a specific genomic region. However, it can be tricky to achieve a robust and reproducible signal with sequential ChIP. Here, we provide an optimized two-step ChIP protocol...
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| Published in: | STAR Protoc |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Elsevier
2021
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8100613/ https://ncbi.nlm.nih.gov/pubmed/33997818 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100504 |
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