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High Efficiency RNA Extraction From Sperm Cells Using Guanidinium Thiocyanate Supplemented With Tris(2-Carboxyethyl)Phosphine

The extraction of high-quality ribonucleic acid (RNA) from tissues and cells is a key step in many biological assays. Guanidinium thiocyanate-phenol-chloroform (AGPC) is a widely used and efficient method to obtain pure RNA from most tissues and cells. However, it is not efficient with some cells li...

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Vydáno v:Front Cell Dev Biol
Hlavní autoři: Roszkowski, Martin, Mansuy, Isabelle M.
Médium: Artigo
Jazyk:Inglês
Vydáno: Frontiers Media S.A. 2021
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8097045/
https://ncbi.nlm.nih.gov/pubmed/33968930
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fcell.2021.648274
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