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Optimization of tris(2-carboxyethyl) phosphine reduction conditions for fast analysis of total biothiols in mouse serum samples

In this study, we investigated suitable conditions for the reduction of disulfides in mouse serum samples by tris(2-carboxyethyl) phosphine (TCEP) for fast analysis of total biothiols. Disulfides were reduced with TCEP, and then, thiols were derivatized with the fluorogenic reagent, ammonium 7-fluor...

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Pubblicato in:Heliyon
Autori principali: Chang, Chunfang, Isokawa, Muneki, Funatsu, Takashi, Tsunoda, Makoto
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 2019
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6517333/
https://ncbi.nlm.nih.gov/pubmed/31193090
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.heliyon.2019.e01598
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