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Optimization of tris(2-carboxyethyl) phosphine reduction conditions for fast analysis of total biothiols in mouse serum samples

In this study, we investigated suitable conditions for the reduction of disulfides in mouse serum samples by tris(2-carboxyethyl) phosphine (TCEP) for fast analysis of total biothiols. Disulfides were reduced with TCEP, and then, thiols were derivatized with the fluorogenic reagent, ammonium 7-fluor...

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Detalhes bibliográficos
Publicado no:Heliyon
Main Authors: Chang, Chunfang, Isokawa, Muneki, Funatsu, Takashi, Tsunoda, Makoto
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6517333/
https://ncbi.nlm.nih.gov/pubmed/31193090
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.heliyon.2019.e01598
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