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Modeling human hepatic steatosis in pluripotent stem cell-derived hepatocytes

This protocol describes the production of hepatocyte-like cells (HLCs) from human pluripotent stem cells and how to induce hepatic steatosis, a condition characterized by intracellular lipid accumulation. Following differentiation to an HLC phenotype, intracellular lipid accumulation is induced with...

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Detalles Bibliográficos
Publicado en:STAR Protoc
Main Authors: Sinton, Matthew C., Meseguer-Ripolles, Jose, Lucendo-Villarin, Baltasar, Drake, Amanda J., Hay, David C.
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2021
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC8091923/
https://ncbi.nlm.nih.gov/pubmed/33997813
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100493
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