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Improvement of Opal Multiplex Immunofluorescence Workflow for Human Tissue Sections

The Opal multiplex technique is an established methodology for the detection of multiple biomarkers in one section. The protocol encompasses iterative single stainings and heating-mediated removal of the primary and secondary antibodies after each staining round, leaving untouched the Opal fluoropho...

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Podrobná bibliografie
Vydáno v:J Histochem Cytochem
Hlavní autoři: Willemsen, Marcella, Krebbers, Gabrielle, Bekkenk, Marcel W., Teunissen, Marcel B.M., Luiten, Rosalie M.
Médium: Artigo
Jazyk:Inglês
Vydáno: SAGE Publications 2021
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8091416/
https://ncbi.nlm.nih.gov/pubmed/33797290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1369/00221554211007793
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