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Improvement of Opal Multiplex Immunofluorescence Workflow for Human Tissue Sections
The Opal multiplex technique is an established methodology for the detection of multiple biomarkers in one section. The protocol encompasses iterative single stainings and heating-mediated removal of the primary and secondary antibodies after each staining round, leaving untouched the Opal fluoropho...
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| Publicado no: | J Histochem Cytochem |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
SAGE Publications
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8091416/ https://ncbi.nlm.nih.gov/pubmed/33797290 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1369/00221554211007793 |
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