Protocol for the generation of pancreatic and hepatic progenitors from human pluripotent stem cells for gene regulatory assays

This protocol describes the detailed procedures for utilizing human pluripotent stem cells (hPSCs) for pancreatic progenitor and hepatic differentiation, followed by the application of hPSC-derived cells in a luciferase reporter-based assay to study gene regulation. The generated hPSC-derived cells...

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Bibliographische Detailangaben
Veröffentlicht in:STAR Protoc
Hauptverfasser: Tan, Lay Shuen, Chew, Resilind Su Ern, Ng, Natasha Hui Jin, Teo, Adrian Kee Keong
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2021
Schlagworte:
Protocol
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8086138/
https://ncbi.nlm.nih.gov/pubmed/33997805
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100471
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