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Protocol for the generation of pancreatic and hepatic progenitors from human pluripotent stem cells for gene regulatory assays

This protocol describes the detailed procedures for utilizing human pluripotent stem cells (hPSCs) for pancreatic progenitor and hepatic differentiation, followed by the application of hPSC-derived cells in a luciferase reporter-based assay to study gene regulation. The generated hPSC-derived cells...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Tan, Lay Shuen, Chew, Resilind Su Ern, Ng, Natasha Hui Jin, Teo, Adrian Kee Keong
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8086138/
https://ncbi.nlm.nih.gov/pubmed/33997805
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100471
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