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Free-Radical Membrane Protein Footprinting by Photolysis of Perfluoroisopropyl Iodide Partitioned to Detergent Micelle by Sonication
We describe a free-radical footprinting approach for integral membrane protein (IMP) that extends significantly the “fast photochemical oxidation of proteins” (FPOP) platform. This new approach exploits highly hydrophobic perfluoroisopropyl iodide (PFIPI) together with tip sonication to ensure effic...
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| Publicado no: | Angew Chem Int Ed Engl |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8083173/ https://ncbi.nlm.nih.gov/pubmed/33751812 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.202014096 |
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