Protocol for RNA-seq library preparation starting from a rare muscle stem cell population or a limited number of mouse embryonic stem cells

It remains challenging to generate reproducible, high-quality cDNA libraries from RNA derived from rare cell populations. Here, we describe a protocol for high-throughput RNA-seq library preparation, including isolation of 200 skeletal muscle stem cells from mouse tibialis anterior muscle by fluores...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Dell’Orso, Stefania, Juan, Aster H., Moiseeva, Victoria, García-Prat, Laura, Muñoz-Cánoves, Pura, Sartorelli, Vittorio
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8079445/
https://ncbi.nlm.nih.gov/pubmed/33937872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100451
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