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Highly Efficient Genome Modification of Cultured Primordial Germ Cells with Lentiviral Vectors to Generate Transgenic Songbirds

The ability to genetically manipulate organisms has led to significant insights into functional genomics in many species. In birds, manipulation of the genome is hindered by the inaccessibility of the one-cell embryo. During embryonic development, avian primordial germ cells (PGCs) migrate through t...

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Publicado en:Stem Cell Reports
Main Authors: Gessara, Ivana, Dittrich, Falk, Hertel, Moritz, Hildebrand, Staffan, Pfeifer, Alexander, Frankl-Vilches, Carolina, McGrew, Mike, Gahr, Manfred
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2021
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC8072032/
https://ncbi.nlm.nih.gov/pubmed/33740464
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2021.02.015
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