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Highly Efficient Genome Modification of Cultured Primordial Germ Cells with Lentiviral Vectors to Generate Transgenic Songbirds

The ability to genetically manipulate organisms has led to significant insights into functional genomics in many species. In birds, manipulation of the genome is hindered by the inaccessibility of the one-cell embryo. During embryonic development, avian primordial germ cells (PGCs) migrate through t...

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Bibliographische Detailangaben
Veröffentlicht in:Stem Cell Reports
Hauptverfasser: Gessara, Ivana, Dittrich, Falk, Hertel, Moritz, Hildebrand, Staffan, Pfeifer, Alexander, Frankl-Vilches, Carolina, McGrew, Mike, Gahr, Manfred
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2021
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8072032/
https://ncbi.nlm.nih.gov/pubmed/33740464
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2021.02.015
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