Protocol for electron microscopy ultrastructural localization of the fusogenic lipid phosphatidic acid on plasma membrane sheets from chromaffin cells

The glycerophospholipid phosphatidic acid (PA) is a key player in regulated exocytosis, but little is known about its localization at the plasma membrane. Here, we provide a protocol for precisely determining the spatial distribution of PA at exocytotic sites by electron microscopy. Using primary bo...

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Dades bibliogràfiques
Publicat a:STAR Protoc
Autors principals: Tanguy, Emeline, Thahouly, Tamou, Royer, Cathy, Demais, Valérie, Gasman, Stéphane, Chasserot-Golaz, Sylvette, Vitale, Nicolas
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2021
Matèries:
Protocol
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8065343/
https://ncbi.nlm.nih.gov/pubmed/33912850
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100464
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